Back استشراب انجذابي Arabic Афинитетна хроматография Bulgarian Afinitní chromatografie Czech Affinitätschromatographie German Cromatografía de inmunoafinidad Spanish Afiinsuskromatograafia Estonian Affiniteettikromatografia Finnish Chromatographie d'affinité French Cromatografía de afinidade Galician Kromatografi afinitas ID
Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance. The specific type of binding interaction depends on the biomolecule of interest; antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid[1] binding interactions are frequently exploited for isolation of various biomolecules. Affinity chromatography is useful for its high selectivity and resolution of separation,[2][3] compared to other chromatographic methods.
- ^ Aizpurua-Olaizola, Oier; Sastre Torano, Javier; Pukin, Aliaksei; Fu, Ou; Boons, Geert Jan; de Jong, Gerhardus J.; Pieters, Roland J. (January 2018). "Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate-based cholera toxin inhibitors". Electrophoresis. 39 (2): 344–347. doi:10.1002/elps.201700207. hdl:1874/362143. PMID 28905402. S2CID 33657660.
- ^ Ninfa, Alexander J.; Ballou, David P.; Benore, Marilee (2009). Fundamental Laboratory Approaches for Biochemistry and Biotechnology (2nd ed.). Wiley. p. 133. ISBN 9780470087664.
- ^ ""Introduction to Affinity Chromatography"". bio-rad.com. Bio-Rad. 14 September 2020. Retrieved 14 September 2020.